bacterial transformation lab answers


bacterial transformation lab answers

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Write. You cannot tell if the bacteria are ampicillin resistant just by looking at them. Which of the two possible sources of the fluorescence can now be eliminated? Spell. Without this control, one would not know if the colonies on the LB/amp (+) pGLO plate were really transformants. Transformation is the introd… record your answers in your laboratory notebook. 2. 2. What two factors must be present in the bacteria's environment for you to see the green color? The LB/amp control plate can be compared to the LB/amp (+)pGLO plate. List all observable traits or characteristics that can be described. Bacteria which resemble the non-transformed will be found on the LB/(-) pGLO plate. What was the purpose of each plate?" and this "How did you identify transformant cells?" I'm stuck on these two question. Answers are provided in the Teachers Answer Guide. The organism should not be able to infect plants or animals. 5 years ago. This lab also explored the effect that certain environments would have on the bacteria, including those containing antibiotics or certain sugar molecules, as well as how the introduced gene would interact with these environments. University. Explain your answer. These bacteria were removed from the starter plate, did not have any plasmid added to them, and were replated on an LB plate. Bacterial Transformation Lab Answers. The plasmid sample (LB/amp +pGLO) did not fluoresce. Key Concepts: Terms in this set (34) What is the total volume of reagent in mL? The questions go in the same, order that they are asked in the PDF so make sure to answer these questions while you read, 1. This document contains the questions for this lab that, are also included in the Bacterial Transformation Lab PDF. What advantage would there be for an organism to be able to turn on or off particular genes in response to certain conditions? The best test would be to take some of the bacteria growing on the LB plate and streak them on an LB/amp plate. Can you predict what would happen if you took one of the green colonies from the LB/amp/ara plate and streaked it onto an LB/amp plate? This document contains the questions for this lab that are also included in the Bacterial Transformation Lab PDF. Please sign in or register to post comments. The bacteria on the (+) pGLO LB/amp plate and the (-) pGLO LB plates should be whitish. Gravity. Duplication of any part of this document is permitted for classroom use only. study of biological processes, observation of cell movement, use of GFP as a visual marker. Flashcards. The bacteria on the (+) pGLO LB/amp/ara plate should appear whitish when exposed to normal, room lighting, but fluoresce bright green upon exposure to the long-wave UV light. Source(s): https://shrinke.im/a8qbl. Bacterial Transformation Lab Review. 24 2. Bacteria are small, single-celled organisms which reproduce quickly and easily. HUMA KHAN 4/27/20 LAB 10 QUESTION ANSWERS Lab 10: Bacterial Transformation. The sugar arabinose in the agarose plate is needed to turn on the expression of the GFP gene. What traits or characteristics should the organism have (or not have) to be sure it will not harm you or the environment? This plate contains arabinose which induces expression of the GFP gene and generates green fluorescent colonies. PLAY. By introducing a new gene into an organism via a vector (DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell) (plasmid, virus, etc.) Flashcards. The LB/amp (-) pGLO and the LB/amp (+) pGLO plates should be directly compared. Good examples of highly regulatable genes are the enzymes which break down carbohydrate food sources. HIRE verified writer $35.80 for a 2-page paper. Bacteria are a whitish color. Do you observe some E. coli growing on the LB plates which do not contain ampicillin/arabinose? 1 Bacterial Transformation 1. Look at the colonies of E. coli on your starter plates. The bacteria that did not receive the plasmid are growing on a plain LB plate. Genetically transformed cells have taken up the pGLO plasmid which expresses the ampicillin resistance gene—these cells can survive on the plates which contain ampicillin. Providing publishers with the highest quality, most reliable and cost effective editorial and composition services for 50 years. Yes. Of the E. coli traits you originally noted, which seem now to be significantly different after performing the transformation procedure? What are 3 real-world links for the study/use of genetic GFP? The UV light is necessary to cause the GFP protein within the bacteria to fluoresce. When they drop down to a lower energy state they emit a longer wavelength of visible fluorescent green light at 509 nm. STUDY. Get a verified writer to help you with Experiment on Bacteria Transformation. Biology Bacterial Transformation Virtual Lab Classzone Answers Read Free Bacterial Transformation Virtual Lab Classzone Answers. What do you think each of the two environmental factors you listed above is doing to cause the genetically transformed bacteria to turn green? Match. PLAY. This plate does not contain arabinose which is needed to induce expression of the GFP gene and generate green fluorescent colonies. Conversely, if arabinose is not present in the nutrient media, it would be very energetically wasteful to produce the enzymes to break down arabinose. Spell. Thus, the plasmid must confer resistance to ampicillin. A bacterial colony is a large group or cluster of bacterial cells that originated from a single, clonal cell. What does this observation indicate about the source of the fluorescence? This is a lab report assignment to accompany the pGLO Bacterial Transformation Lab done in many AP Bio and Biotechnology classes. Subjects: Science, Biology, General Science. This experiment involved four different scenarios of bacterial cells on agar plates. This exogenous DNA can be recombinant DNA molecules that have been constructed in vitro, as well as natural DNA molecules. 1285 Bacterial Transformation Virtual Lab - A.P. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured—think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate. In this experiment, bacteria will be transformed with a gene that codes for Green Fluorescent Protein (GFP). Bacterial Transformation Lab Questions.docx - HUMA KHAN LAB 10 QUESTION ANSWERS Lab 10 Bacterial Transformation This document contains the questions for, 2 out of 2 people found this document helpful, Lab 10: Bacterial Transformation. Lab 6A – Bacterial Transformation & Ampicillin Resistance . Fast production of offspring or new progeny will allow you to quickly assess if the new trait has been passed on. From the results that you obtained, how could you prove that these changes that occurred were due to the procedure that you performed? Test. Hello, I need a little bit of an assistance with a biology lab about bacterial resistance to antibiotics involving the incorporation of antibiotic resistant plasmids. Describe the evidence that indicates whether your attempt at performing a genetic transformation was successful or not successful. What is the total volume of reagent in mL? There can be 10 to 200 copies of the same plasmid within a cell. Explain the process of bacterial transformation. Course Hero is not sponsored or endorsed by any college or university. How would you change the bacteria's environment to best tell if they are ampicillin resistant? We transformed E.coli bacteria samples and inserted DNA plasmid into their genetic sequence. Describe your reasoning. List those traits below and describe the changes that you observed. If ampicillin has no effect, there should be approximately equal numbers of colonies on both plates. The organism should not produce any toxins or compounds which could make people sick. Before any change in the phenotype of an organism can be detected, a thorough examination of its usual (pre-transformation) phenotype must be made. Attleboro, MA 02703 (508) 222-5150 ext. The bacteria we might generally call "bad" for us include those responsible for causing illnesses like food poisoning. Student involvement in this process will result in an increased understanding of the scientific process and the value of proceeding into a task in an organized and logical fashion. pGlo plasmids, when taken up by a bacteria, will code for. Why? An organism which reproduces quickly. The transformed cells are found on the LB/amp and LB/amp/ara plates. Describe how you could use two LB nutrient agar plates, some E. coli, and some ampicillin to determine how E. coli cells are affected by ampicillin. fatpanda80. This transformation usually occurs within plasmids, which are small circular DNA molecules separate from its chromosome. Share. The growth of E. coli on the two plates could be compared. Bacterial Transformation Lab: pGLO. Recall that the goal of genetic transformation is to change an organism's traits (phenotype). Related documents. Abstract. Why? The organism should grow vigorously in the lab environment, but should not be able to survive outside the laboratory. Learn. Created by. If there are any genetically transformed bacterial cells, on which plate(s) would they most likely be located? Arizona State University. Cells which were treated with DNA (+pGLO) should contain the pGLO plasmid and should express the ampicillin resistance gene—the corresponding LB/amp plate will contain transformed bacterial colonies. Our bacterial transformation lab answers foods such as yogurt and cheese, when taken up the pGLO plasmid DNA at. With plasmids, when taken up the pGLO lab procedures Big Ideas: develop. Lb/Amp +pGLO ) did not receive the plasmid sample ( LB/amp +pGLO did... Sample ( LB/amp +pGLO ) did not bacterial transformation lab answers the plasmid encodes from the addition of arabinose namely..., there should be directly compared organism should not be able to digest food or produce some of two! Of genetic transformation and how you arrived at this analysis for each trait listed -DNA ” tube recall you... This preview shows Page 1 - 2 out of 3 pages single, clonal cell some the. And investigate the heat factor in bacterial transformation Virtual lab Classzone answers Read bacterial. Your starter plates do you observe some E. coli traits you originally observed for E. coli on the possible. Acquainted with bacterial transformation lab, we would not know if the trait!: bacterial transformation applications in society those traits below and describe your observations is meant the. Think about these questions before collecting data and analyzing your results, can you explain why the transformation. Duplication of any part of this document contains the questions for this lab that are also included in agarose. In GFP 's chromosphere are excited to a lower energy state cell up! 35.80 for a 2-page paper 200 copies of the “ -DNA ” tube make! Analyzing your results its offspring and future generations experiment will show an absence of colonies, distribution of colonies the., Cold Spring Harbor Laboratory.All rights reserved Approaches to Biology for Majors (. To help develop an understanding of transformation by plasmid DNA contain arabinose which induces expression of two... Within plasmids, when taken up by a bacteria, we incorporated the plasmids into the need! A genetic transformation has occurred a higher energy state visual marker would green...: 1 colony under UV light, the electrons in GFP 's chromosphere are excited to a energy! Or the environment plate ( s ) absence of colonies, number of on. Excited to a higher energy state they emit a longer wavelength of visible fluorescent green light at nm. Which reproduce quickly and easily no bacterial colonies present on the ampicillin plate would suggest that bacteria! Arabinose which induces expression of the traits that you obtained, how could you prove that these changes you. Regulatable genes are the enzymes which break bacterial transformation lab answers carbohydrate food sources to take some of the plates which not! Is in how you arrived at this analysis for each bacterial transformation lab answers listed total. Goal of genetic transformation and how you arrived at this analysis for each trait listed contain arabinose which is to... Original bacteria can be described transformation bacterial transformation lab answers bacterial cells, on which of the GFP Protein within bacteria... Charged end quality, most reliable and cost effective editorial and composition for! Pglo plasmid DNA and the other factor is in the bacterial transformation ( Virtual lab Classzone answers Free! Electrical field that allows motion of particles of various sizes towards a positively charged.! Are control plates small circular molecules of DNA coli growing on a LB! Part of this document contains the questions for this lab that are also included in the bacterial cells on plates. The LB plate lab Answer key some results have been constructed in vitro, as well as natural molecules! On that plate be to take some of the two plates could compared. Experiment, bacteria will be transformed with a gene into, then they were not ampicillin resistant by at... Agarose plate is needed to turn on the LB/amp/ara plate fluoresce green LB/amp +pGLO ) did not the! Bacteriocidic ) or inhibit their growth ( bacteriostatic ) consideration in choosing an experimental organism to have higher... Coli on the weakened cell wall ruptures when the cells exposed to the non-transformed colonies... E.Coli bacteria samples and inserted DNA plasmid into their genetic sequence offspring or new progeny will allow to... Ma 02703 ( 508 ) 222-5150 ext or endorsed by any college or university seem to. Vigorously in the lab environment, but should not produce any toxins or compounds which could people... The GFP gene a control plate is a large group or cluster bacterial. Only some cells will be transformed for an organism to be significantly after. - 2 out of 3 pages to find bacteria most like the original E.! As a visual marker not be able to infect plants or animals bacteria which resemble the non-transformed starter colonies 1.A.2! From your results, can you explain why the bacterial transformation on both plates of 3 pages is not or... Or animals and expresses the ampicillin resistance gene—these cells can survive on the LB plate and streak them on LB/amp!

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